MSc in Plant Biology – Plant Health

Faculty of Sciences, Angers | 2022 – 2024

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Overview

This Master’s program provided specialized training in Plant Health, combining advanced plant physiology with the study of (a)biotic interactions. The curriculum also included an introductory exposure to Data Science—covering basic programming and modeling—which helped me start bridging the gap between biological experimentation and computational analysis.

Core Competencies

Plant Health & Pathology

The core of the degree focused on the complex interactions between plants, microbiota, and their environment.

  • Pathology & Immunology: I studied the biology of bioaggressors (detection and diversity), host-parasite interactions, the mechanisms of (re)-emerging diseases, and plant innate immunity signaling pathways—pattern-triggered immunity (PTI) and effector-triggered immunity (ETI).

  • Microbial Ecology: Analysis of plant-associated microbial communities (microbiota) and the balance between parasitic and mutualistic strategies. This included studying Synthetic Communities (SynComs) to model microbiome interactions.

  • Holobiont Concept: Considered the plant and its associated microbiota as an integrated functional unit (host + microbiome) shaping health, stress responses, and disease dynamics.

  • Protection Strategies: I examined both conventional and genetic methods for plant protection, alongside the study of secondary metabolites involved in defense.

Genetics, Genomics & Physiology

I acquired a systemic view of plant development and improvement.

  • Omics & Breeding: Coursework covered Plant Genomics and Genetics, with a specific focus on quantitative methods like QTL mapping and GWAS (Genome-Wide Association Studies).
  • Physiology: I deepened my understanding of hydro-mineral nutrition, crop development, and signaling pathways in cultivated plants.
  • Product Quality: Assessment of plant product quality and biomass elaboration.

Data Science & Modeling

This program provided foundational training in computational and statistical approaches.

  • Programming & ML: Acquired strong foundations in R programming and theoretical concepts of Machine Learning.

  • Bioinformatics: Theoretical introduction to omics concepts (e.g., RNA‑seq), not full data-management or analysis pipelines.

  • Statistics: Advanced experimental design and statistical analysis.

Key Academic Projects

Beyond theoretical coursework, the program emphasized practical application through hands-on projects:

Experimental Research Project

Designed and executed an independent experimental protocol during my first year of Master’s, which marked my first hands-on experience in a research laboratory setting.

  • Project Title: Realization of a Detached Leaf Assay to evaluate the qualitative resistance of roses to the Diplocarpon rosae fungus
  • Duration: 7 days | Partner: Lisa Gelé
  • Laboratory: IRHS (Institut de Recherche en Horticulture et Semences), GDO team
  • Supervisors: Laurine Lambelin, Sophie Paillard
Grant Writing Simulation (ANR Project)

Simulated the complete process of scientific grant writing for the French National Research Agency (ANR). This involved bibliographic research, experimental planning, and scientific communication.

Project Title: RP-SCREEN - Characterization of cell surface receptors involved in the recognition of phytocytokines through the use of a novel high-throughput cell screening method employing CRISPRa.

Project Overview: This fundamental research project aimed to characterize membrane receptors involved in the perception of secreted peptides (phytocytokines) in the model plant Arabidopsis thaliana. The proposal addressed the challenge of identifying receptor-ligand interactions using a novel high-throughput cell screening approach based on CRISPR activation (CRISPRa), recently developed for human cells. This innovative method would allow comprehensive exploration of receptor diversity and overcome the limitations of traditional genetic and biochemical approaches, which are time-consuming and may not fully represent in vivo interactions.

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Applied Deep Learning and computer vision techniques to develop a model capable of identifying and classifying pathogens based on leaf imagery.

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Presentations & Vulgarisation

Throughout this Master’s program, I delivered several scientific presentations on specialized topics in plant biology, genetics, and pathology. These oral communications enhanced my ability to synthesize complex information and communicate scientific concepts effectively.

Presentation on the study: “Wheat microRNA1023 suppresses invasion of Fusarium graminearum via targeting and silencing FGSG_03101”. Explores how plant microRNAs can act as defense molecules by directly targeting and silencing pathogen genes, demonstrating cross-kingdom RNA interference.

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Presentation on Quantitative Trait Loci (QTL) mapping strategies and their application in plant breeding and trait improvement.

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Presentation on the study: “The Xanthomonas type-III effector XopS stabilizes CaWRKY40a to regulate defense responses and stomatal immunity in pepper (Capsicum annuum)”. Analyzes how bacterial effectors manipulate plant transcription factors to suppress defense mechanisms and facilitate infection.

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Presentation on the study: “Rhizosphere microbiome mediates systemic root metabolite exudation by root-to-root signaling”. Examines how plant roots communicate through the rhizosphere microbiome, leading to systemic changes in secondary metabolite exudation and plant-microbe interactions.

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Laboratory Reports Examples

Throughout the program, I completed numerous practical laboratory sessions that reinforced theoretical concepts and developed hands-on experimental skills. Below are two representative lab reports demonstrating my ability to design experiments, analyze data, and communicate scientific findings.

Lab Report: Screening of plant protection and/or biostimulation products based on Trichoderma

This practical work focused on evaluating the efficacy of Trichoderma-based biocontrol agents as alternatives to chemical pesticides. The study assessed the protective and biostimulant effects of different Trichoderma strains on plant health and disease resistance.

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Lab Report: Influence of host genotype and terroir on fungal microbiota assembly in bean seeds

This practical work exploited results from the study by Barret et al. (2015) to evaluate the relative influence of host genotypes and terroir on seed microbiota assembly. Four bean cultivars were multiplied over two consecutive years in two farms (Brittany and Luxembourg), with three replicates per condition. Microbial community structure was assessed by metabarcoding of the 16S rRNA gene (bacteria) and ITS1 region (fungi). This report focuses on fungal microbiota analysis, demonstrating bioinformatic and statistical methods for high-throughput sequencing data.

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Professional Research Experience

Research Internships

This Master’s program included two significant research internships that allowed me to apply theoretical knowledge in real laboratory settings and develop practical research skills.

MSc Research Intern – Plant-Pathogen Interactions & Phytocytokines

May – July 2023 | UMR 1345 IRHS, Angers

Phenotyped Arabidopsis thaliana resistance to Alternaria brassicicola in phytocytokine mutants identified via transcriptomics, as part of the ANR STRESS-PEPT project.

Supervised by Thomas Guillemette, Philippe Grappin (FUNGISEM team) & Sébastien Aubourg (BIDEFI team)

MSc Research Intern – Plant Phenotyping & Growth-Defense Trade-offs

Jan. – June 2024 | UMR 1345 IRHS, Angers

Developed semi-automated phenotyping of apple trees using robotic imaging to analyze growth-defense trade-offs, including monitoring of growth and resistance to Erwinia amylovora.

Supervised by Florent Pantin, Bao-Huynh Nguyen & Romain Larbat (RESPOM team)


Complementary Bioinformatics Training

Motivation for MSc in Bioinformatics

This Master’s in Plant Health gave me strong theoretical foundations in statistics, machine learning concepts, introductory R programming, genetics/genomics and phenotyping. However, it did not train me to manage and analyze omics datasets in practice (e.g., RNA‑seq, metabolomics).

To develop additional research competencies and bridge the gap between experimental biology and computational data analysis, I pursued a specialized MSc in Bioinformatics applied to Biomedical and Health Sciences. This complementary training not only equipped me with the advanced bioinformatics skills necessary to independently conduct modern biological research—where high-throughput data generation and computational analysis have become essential—but also allowed me to expand my scope toward human biology and medicine, a long-standing interest of mine.

Through the CoCoBi minor (Complementary Skills in Bioinformatics), I gained direct admission to this intensive M2 program. I developed expertise in the analysis, interpretation, and visualization of massive omics data, with applications spanning diagnostics, biomedical research, and fundamental biology. I also strengthened my skills in statistics and machine learning, and learned rigorous coding practices following the FAIR principles.

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